Does balsamic vinegar go bad?
By
Dr Rafael Frascino Cassaro (PhD)
| Reviewed by
Dr Anny Manrich (PhD)
The contents of this article are fact-based except otherwise stated within the article.
Author bio
Dr Rafael Frascino Cassaro PhD is a Chemist with expertise in Food Technology, extraction of essential oils from food sources, organocatalysis, extraction by supercritical CO2 and organic chemistry. He writes and reviews content on these topics.
Dr Rafael Frascino Cassaro’s Highlights:
- PIPE-FAPESP Project Coordinator for the company Bioativos Naturais Ltda involving the extraction of essential oils from food sources, with supercritical CO2.
- PhD in Chemistry with research in supercritical fluid reactivity.
- Bachelor of Environmental Chemistry at University of São Paulo State (UNESP)/Brazil
Professional Experience:
Dr Rafael Frascino Cassaro’s Experience:
Dr Rafael coordinated a PIPE-FAPESP Project (Company Research and Innovation Project) for the company Bioativos Naturais Ltda, involving the extraction of essential oils with supercritical CO2 from food sources such as turmeric, ginger, hops, orange bagasse, pepper rose, cupuaçu pie, among others.
Dr Rafael has worked on a project, involving studies of the influence of pre-treatment on the recovery of crude food extracts by extraction with supercritical CO2 (Supercritical Fluid Extraction or SFE) and with pressurized liquids (ethanol and water) for food sources, aimed at obtaining oleoresins; determination of global yield and chemical composition of crude extracts. As a postdoctoral fellow for the Food Engineering department at the University of Campinas/Brazil
Dr Rafael Frascino Cassaro has worked as a substitute Professor at the University of São Paulo State (UNESP) on General Chemistry and Thermodynamics II disciplines for the Food Engineering course.
Education:
- 2009 Bachelor in Environmental Chemistry at the State University of São Paulo, Brazil
- 2015 PhD in Chemistry with research in supercritical fluid reactivity University of São Paulo, Brazil
- 2017 Post Doctoral in Food Engineering Research: Influence of Biomass Pre-Treatment on Supercritical CO2 Extraction: Preservation of High Added Value Assets and Reduction of Process Costs at the State University of Campinas, Brazil
The main publications of Dr Rafael Frascino Cassaro are:
RC Bazito, RF CASSARO, LC OLIVEIRA, RA Gariani, CAO Nascimento. (2013). Proline derivatives as organocatalysts for the aldol reaction in conventional and non-conventional reaction media. Green Processing and Synthesis 2, 43-50
TS Bastos, SB Rodriguez Reartes, MS Zabaloy, RF Cassaro, RC Bazito. (2019).Phase Behavior for the System Carbon Dioxide plus p-Nitrobenzaldehyde: Experimental and Modeling JOURNAL OF CHEMICAL AND ENGINEERING DATA 64 (5), 2116-2125
GH Sakae, LM Takata, AS Paulino, RC Bazito, RF Cassaro, C Princival, .(2013). A high enantioselective Proline-based helical polymer catalyst for aldol type reaction. Blucher Chemistry Proceedings 1 (2), 214-214
You can view some of Dr Rafael’s work below and links to his professional profile.
Research Gate: https://www.researchgate.net/profile/Rafael-Cassaro-2
Fapesp: https://bv.fapesp.br/pt/pesquisador/680718/rafael-frascino-cassaro/
Google Scholar: https://scholar.google.com/citations?user=ToO7HIcAAAAJ&hl=en
Linkedin: https://www.linkedin.com/in/rafael-frascino-cassaro-4a156a37/?originalSubdomain=br
ORCID: https://orcid.org/0000-0002-3729-2858
Reviewer bio
Dr Anny Manrich PhD is a food Engineer with expertise in Food Technology, Natural Polymers, Edible Films, Enzymes, and Nanotechnology. She writes and reviews content on these topics.
Dr Anny Manrich’s Highlights:
- Research and Technology at the Brazilian Agricultural Research Corporation
- PhD in Chemical Engineering with a focus on Biochemistry at the Federal University of Sao Carlos/ Brazil and a one-year scholarship at the Technical University of Munich/ Germany
- Bachelor of Food Engineering at the University of Campinas/ Brazil and a one-year scholarship at the Technical University of Munich/ Germany
“To solve a problem, global vision and multifactorial understanding are necessary. Therefore, in addition to expertise, one should seek multidisciplinary thinking connected with science and reality” – Dr Anny Manrich, PhD.
Professional Experience:
Dr Anny Manrich’s Experience Joining the Brazilian Agricultural Research Corporation, as soon as she completed her doctorate,
Dr Anny Manrich has worked on several projects, including the more than three-year partnership project with BRF, a major food producer in Brazil. As a postdoctoral fellow.
Dr Anny Manrich has also contributed to several business consultancies and research projects of the National Nanotechnology Laboratory System in areas such as food technology, fibres, films and coatings and Nanotechnology; in a very determined way, having a great team relationship, being creative and committed.
Growing concerns about the safe introduction of nanomaterials into today’s life emphasises the need to create regulatory documentation in front of characterising, using and testing them. Dr Anny Manrich worked for two years on a characterization project for nanoscale materials, with the aim of exploring their possible health effects.
Despite not having specific academic training in packaging or polymeric films, Dr Anny Manrich works at the Brazilian Agricultural Research Corporation in areas of edible and biodegradable films produced from agricultural waste and in the development of films with greater resistance to water, having articles published in renowned scientific journals, which demonstrates her multidisciplinary understanding and creativity.
In addition, she worked for four years as a consultant to a food company to develop a line of snacks that are healthy and that add functional ingredients, physiologically active compounds that bring health benefits, made from fruits and vegetables, enabling diet improvement, disease prevention and reduction of nutritional deficiencies.
Dr Anny Manrich participated as a member of the examination board for two Master’s exams and one PhD exam at the Department of Chemical Engineering of the Federal University of São Carlos.
Education:
- 2001 Bachelor in Food Engineering at the State University of Campinas, Brazil
- 1999 One year scholarship at the Technical University of Munich
- 2004 Master in Chemical Engineering at the Federal University of São Carlos, Brazil
- 2012 PhD in Chemical Engineering at the Federal University of São Carlos, Brazil
- 2010 One year scholarship at the Technical University of Munich
The main publications of Dr. Anny Manrich are:
Articles
Manrich, A., Moreira, F. K., Otoni, C. G., Lorevice, M. V., Martins, M. A., & Mattoso, L. H. (2017). Hydrophobic edible films made up of tomato cutin and pectin. Carbohydrate Polymers, 164, 83-91.
Mendes, J. F., Norcino, L. B., Martins, H. H. A., Manrich, A., Otoni, C. G., Carvalho, E. E. N., … & Mattoso, L. H. C. (2020). Correlating emulsion characteristics with the properties of active starch films loaded with lemongrass essential oil. Food Hydrocolloids, 100, 105428.
Norcino, L. B., Mendes, J. F., Natarelli, C. V. L., Manrich, A., Oliveira, J. E., & Mattoso, L. H. C. (2020). Pectin films loaded with copaiba oil nanoemulsions for potential use as bio-based active packaging. Food Hydrocolloids, 106, 105862.
Manrich, Anny, et al. Immobilization of trypsin on chitosan gels: Use of different activation protocols and comparison with other supports. International Journal of Biological Macromolecules 43.1 (2008): 54-61.
Manrich, Anny; Komesu, Andrea ; Adriano, Wellington Sabino; Tardioli, Paulo Waldir ; Giordano, Raquel Lima Camargo . Immobilization and Stabilization of Xylanase by Multipoint Covalent Attachment on Agarose and on Chitosan Supports. Applied Biochemistry and Biotechnology, v. 161, p. 455-467, 2010.
Mendes, J. F., Martins, J. T., Manrich, A., Neto, A. S., Pinheiro, A. C. M., Mattoso, L. H. C., & Martins, M. A. (2019). Development and physical-chemical properties of pectin film reinforced with spent coffee grounds by continuous casting. Carbohydrate polymers, 210, 92-99..
Milessi, T. S., Kopp, W., Rojas, M. J., Manrich, A., Baptista-Neto, A., Tardioli, P. W., … & Giordano, R. L. (2016). Immobilization and stabilization of an endoxylanase from Bacillus subtilis (XynA) for xylooligosaccharides (XOs) production. Catalysis Today, 259, 130-139.
Mendes, J. F., Norcino, L. B., Manrich, A., Pinheiro, A. C. M., Oliveira, J. E., & Mattoso, L. H. C. (2020). Development, physical‐chemical properties, and photodegradation of pectin film reinforced with malt bagasse fibers by continuous casting. Journal of Applied Polymer Science, 137(39), 49178.
Mendes, J. F., Martins, J. T., Manrich, A., Luchesi, B. R., Dantas, A. P. S., Vanderlei, R. M., … & Martins, M. A. (2021). Thermo-physical and mechanical characteristics of composites based on high-density polyethylene (HDPE) e spent coffee grounds (SCG). Journal of Polymers and the Environment, 29, 2888-2900..
Mendes, J. F., Norcino, L. B., Martins, H. H., Manrich, A., Otoni, C. G., Carvalho, E. E. N., … & Mattoso, L. H. C. (2021). Development of quaternary nanocomposites made up of cassava starch, cocoa butter, lemongrass essential oil nanoemulsion, and brewery spent grain fibers. Journal of Food Science, 86(5), 1979-1996.
Manrich, A., Martins, M. A., & Mattoso, L. H. C. (2021). Manufacture and performance of peanut skin cellulose nanocrystals. Scientia Agricola, 79.
Nascimento, V. M., Manrich, A., Tardioli, P. W., de Campos Giordano, R., de Moraes Rocha, G. J., & Giordano, R. D. L. C. (2016). Alkaline pretreatment for practicable production of ethanol and xylooligosaccharides. Bioethanol, 2(1)..
Manrich, Anny, de Oliveira, J. E., Martins, M. A., & Mattoso, L. H. C. Physicochemical and Thermal Characterization of the Spirulina platensis. J. Agric. Sci. Technol. B, v. 10, p. 298-307, 2020.
Book Chapter
Terra, I. A. A., Aoki, P. H., Delezuk, J. A. D. M., Martins, M. A., Manrich, A., Silva, M. J., … & Miranda, P. B. (2022). Técnicas de Caracterização de Polímeros. Nanotecnologia Aplicada a Polímeros, 614.
Conference Papers
Ferreira, L. F., Luvizaro, L. B., Manrich, A., Martins, M. A., Júnior, M. G., & Dias, M. V. (2017). Comparação da estabilidade de suspensões poliméricas de amido/tocoferol e quitosana/tocoferol. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 14., 2017, Águas de Lindóia, SP.
Manrich, A., Hubinger, S. Z., & Paris, E. C. (2017). Citotoxicidade causada por nanomateriais: avaliação do micronúcleo. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais… São Carlos: Embrapa Instrumentação, 2017. p. 655-658.
Manrich, Anny, et al. Immobilization and Stabilization of Xylanase by multipoint covalent attachment on Glyoxyl Agarose Support. The 31st Symposium on Biotechnology for Fuels and Chemicals. 2009.
Manrich, Anny, et al. Application of immobilized xylanase on hydrolysis of soluble wood hemicelluloses after using microwave and organosolv pre-treatments. The 32nd Symposium on Biotechnology for Fuels and Chemicals. 2010.
You can view some of Dr Anny’s work below and links to her professional profile.
Research Gate: https://www.researchgate.net/profile/Anny-Manrich-2
Scopus: https://www.scopus.com/authid/detail.uri?authorId=23103497100
Google Scholar: https://scholar.google.com/citations?hl=en&user=Ea9qpr0AAAAJ
Linkedin: https://br.linkedin.com/in/anny-manrich-20693129
In this brief guide, we will address the question, “does balsamic vinegar go bad” as well as other questions pertaining to the subject at hand like how much time does balsamic vinegar take to go bad or does its acidity change over time. Moreover, we will also discuss some of the storage practices needed to prolong the shelf life of balsamic vinegar.
Does balsamic vinegar go bad?
Balsamic vinegar rarely ever goes bad. It is only when there is an addition of contaminants that it does so.
Vinegar is traditionally the product of the acetous fermentation of dilute alcoholic solutions. It is produced microbiologically from natural alcoholic solutions or by dilution of acetic acid,. by the action of acidophilic bacteria, resulting in a very low pH.(1)
Traditional balsamic vinegar (TBV) is homemade vinegar produced in Italy, by traditional method in surface culture fermentation. The raw material is cooked grapes with pH values of 2.3–3.2. As with other vinegars, it is obtained by a two-stage fermentation process. (2)
This makes it unfavorable for more bacteria to grow because of the competition that takes place inside the medium.
Some studies have also recently highlighted that yeasts metabolism can affect balsamic vinegar chemical properties. (8) Contamination with toxins derived from grape is also possible (9)
What are the health implications of consuming contaminated vinegar?
Especially vinegar made from fruits collected by consumers from their own orchards contains much higher amounts of toxins derived from these fruits.
These toxins may cause damage to vital organs and systems including the liver, kidneys, and the immune system.
Another negative impact is seen in the gastrointestinal system, disrupting intestinal barrier function. In addition, it may cause a disruption in the balance between intestinal microbiota.(9)
What changes may occur over time in balsamic vinegar?
Like other kinds of vinegar, balsamic vinegar does not expire, some organoleptic or aesthetic changes might occur over the course of time that may be the result of exposure to light and air, these changes are mainly due to improper storage (2,3, 4, 6):
- Balsamic vinegar may get hazy or cloudy due to deposition of some sediments, but they can be removed after filtration and vinegar are good to use again.
- Balsamic vinegar that has been stored for a longer duration develops a slimy substance particularly known as ‘mother’ because it can be used to make a new batch of vinegar.
- By the time, the oxygenation process occurs in balsamic vinegar that may affect its odor and flavor that can become deeper, sour and acidic.
- Like other food products, vinegars also suffer oxidation after being opened. Oxidation processes promote a series of chemical and enzymatic reactions that alter vinegar. Therefore, oxygen is the main cause of vinegar quality deterioration.
- All aromatics (grape, alcohol, yeast and nuruk flavor), except caramel, decreased after time compared to their initial values. This result may be due to the evaporation of volatile components in vinegars during storage.
Does the acidity of vinegar change?
The pH tends to decrease during the storage period, mainly due to the amount of organic or total acids present in the vinegar.
The change in total acidity with storage tends to increase in non-sterilized products, as acetic acid produced by the action of acetic acid bacteria in non-sterile products tend to determine the total acid content.(6)
How to store balsamic vinegar?
Storing balsamic vinegar is pretty similar to storing other kinds of vinegar, like rice, white or red wine vinegar. Proper storage helps in reducing food waste and maximizing food quality by delaying their deterioration and decomposition processes.
Never buy balsamic vinegar that’s in a clear bottle as the light will cause the balsamic to deteriorate more rapidly. Balsamic vinegar should be stored in an airtight properly sealed container to protect it from oxidative changes. If exposed to air, it will cause evaporation hence, resulting in loss of flavor.
Balsamic vinegar should be placed at the dry place at room temperature. Direct sunlight and heat could degrade the vinegar much more quickly. Balsamic vinegar should be kept away from heat sources and light at some dark place to extend its shelf life.
Does balsamic vinegar need refrigeration or freezing?
Balsamic vinegar is best stored at room temperature as it is highly acidic in nature
The organic acids in vinegar (mainly acetic acid) are capable of passing through cell membranes, inhibiting the proliferation of many types of pathogenic bacteria in different foods
Thus, the product is safe from microbial spoilage therefore it is known as self-preserving.(5)
Other FAQs about Vinegar which you may be interested in.
How long does Balsamic vinegar last?
Conclusion
In this brief guide, we have addressed the question, “does balsamic vinegar go bad” as well as other related questions pertaining to the subject at hand like how much time does balsamic vinegar take to go bad or does its acidity changes over time and what are some of the storage practices needed to prolong the shelf life of balsamic vinegar.
References
- M. Plessi, VINEGAR, Encyclopedia of Food Sciences and Nutrition (Second Edition), Academic Press, 2003, 5996-6004,
- Maria Gullo, Paolo Giudici, Acetic acid bacteria in traditional balsamic vinegar: Phenotypic traits relevant for starter cultures selection, International Journal of Food Microbiology, 125,, 2008,46-53,
- Marlene Geiger, Vinegar Shelf Life and Safety, Iowa State University, Answer Line, 2021
- V. Andreou, M. Giannoglou, M.Z. Xanthou, M. Metafa, G. Katsaros, Aging acceleration of balsamic vinegar applying micro-oxygenation technique, Food Chemistry, 419, 2023.
- M.C. Garcia-Parrilla, M.J. Torija, A. Mas, A.B. Cerezo, A.M. Troncoso, Vinegars and Other Fermented Condiments, Fermented Foods in Health and Disease Prevention, 2017, 577-591,
- Minjeong Kang, Jung-Heun Ha, Youngseung Lee, Physicochemical properties, antioxidant activities and sensory characteristics of commercial gape vinegars during long-term storage, Food Sci. Technol, Campinas, 40(4): 909-916, 2020.
- Christine Rukavena, 40 Balsamic-Kissed Recipes, Taste of Home website 2023.
- L. Solieri, P. Giudici Yeasts associated to Traditional Balsamic Vinegar: Ecological and technological features, International Journal of Food Microbiology 125, 36–45, 2008.
- Z.D. Heperkan et al, Unexpectedly high patulin contamination and co-occurrence of ochratoxin A in homemade vinegar, Food Control 148, 2023.
